Features of classical apoptosis in unfertilized Xenopus eggs. Freshly squeezed eggs obtained after hCG injection were dejellied and placed into OR-2 buffer over the indicated times. Cytochrome c release (a), caspase 3 activation (b), apoptotic nuclear morphology (c), and quantification of morphology scores (d) are shown. Immunoblotting with anti-β tubulin (lower panel in (a)) represents the loading control. The upper panel in (b) shows blotting of egg extracts with anti-caspase 3 antibody, whereas the lower panel presents data of the fluorescent caspase 3 assay, carried out as described in "Methods". Bars in panel (b) indicate SD of five to eight measurements using eggs obtained from three different female frogs. About one hundred nuclei were observed in (c) and (d).
Tokmakov et al. BMC Cell Biology 2011 12:56 doi:10.1186/1471-2121-12-56