Open Access Highly Accessed Research article

Unfertilized frog eggs die by apoptosis following meiotic exit

Alexander A Tokmakov12*, Sho Iguchi2, Tetsushi Iwasaki2 and Yasuo Fukami12

Author Affiliations

1 Research Center for Environmental Genomics, Kobe University, Rokko dai 1-1, Nada, Kobe 657-8501, Japan

2 Graduate School of Science, Kobe University, Rokko dai 1-1, Nada, Kobe 657-8501, Japan

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BMC Cell Biology 2011, 12:56  doi:10.1186/1471-2121-12-56

Published: 23 December 2011

Additional files

Additional file 1:

Figure S1. Degradation of unfertilized jelly-coated Xenopus eggs deposited into water. (a) Caspase activation and (b) egg diameter. Data in panel (a) are means ± SD of three measurements, data in panel (b) were obtained by measuring five eggs.

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Additional file 2:

Figure S2. Degradation of unfertilized jelly-coated Xenopus eggs deposited into OR-2 media. (a) Caspase activation and (b) egg diameter. Data in panel (a) are means ± SD of three measurements, data in panel (b) were obtained by measuring three to five eggs.

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Additional file 3:

Figure S3. Degradation of unfertilized dejellied Xenopus eggs deposited into DB buffer. (a) Changes in egg morphology, (b) MAPK dephosphorylation, (c) caspase activation, and (d) egg diameter. Data in panel (d) were obtained by measuring five eggs.

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Additional file 4:

Figure S4. Apoptotic degradation of roscovitine-treated Xenopus eggs. Freshly squeezed dejellied eggs were placed into OR-2 buffer and treated with 50 μM roscovitine. Egg morphology (a), Mos, cyclin B2 levels and MAPK activation state (b), caspase 3 activity (c), intracellular ATP content (d), ADP/ATP ratio (e), and egg diameter (f) have been monitored at the indicated times. Bars in panel (f) represent SD of the mean obtained by measurement of seven eggs.

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