Table 1

Primer sequences used in RT-PCR and Quantitative-PCR

Gene

Sequences

Product Size

Indication


Oct4

Forward: 5'-GGCGTTCTCTTTGGAAAGGTGTTC-3'

Reverse: 5'-CTGGAACCACATCCTTCTCT-3'

312bp

Pluripotency

Sox2

Forward: 5'-CACAACTCGGAGATCAGCAA-3'

Reverse: 5'-CTCCGGGAAGCGTGTACTTA-3'

190bp

Pluripotency

Nanog

Forward: 5'-AAGCAGAAGATGCGGACTGT-3'

Reverse: 5'-GTGCTGAGCCCTTCTGAATC-3'

232bp

Pluripotency

α-MHC

Forward: 5'-CTGCTGGAGAGGTTATTCCTCG-3'

Reverse: 5'-GGAAGAGTGAGCGGCGCATCAAGG-3'

301bp

Cardiomyocyte

ALCAM

Forward:5'-CTTGCACAGCAGAAAACCAA-3'

Reverse: 5'-TAGACGACACCAGCAACGAG-3'

190bp

Cardiogenesis

PCAM-1

Forward: 5'-TGCAGGAGTCCTTCTCCACT-3'

Reverse: 5'-ACGGTTTGATTCCACTTTGC-3'

245bp

Early mesoderm

ANF

Forward: 5'-TGATAGATGAAGGCAGGAAGCCGC-3'

Reverse:5'-GGATTGGAGCCCAGAGTGGACTAGG-3'

203bp

Cardiomyocyte

GAPDH

Forward: 5'-AACTTTGGCATTGTGGAAGG-3'

Reverse: 5'-ACACATTGGGGGTAGGAACA-3'

223bp

Housekeeping gene


Shafa et al. BMC Cell Biology 2011 12:53   doi:10.1186/1471-2121-12-53

Open Data