Open Access Highly Accessed Research article

Impact of stirred suspension bioreactor culture on the differentiation of murine embryonic stem cells into cardiomyocytes

Mehdi Shafa1, Roman Krawetz2, Yuan Zhang1, Jerome B Rattner13, Anna Godollei1, Henry J Duff4 and Derrick E Rancourt1*

Author Affiliations

1 Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, AB, Canada, T2N 4N1

2 Department of Surgery, Faculty of Medicine, University of Calgary, Calgary, AB, Canada

3 Department of Cell Biology and Anatomy, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada

4 Department of Cardiac Sciences, Faculty of Medicine, University of Calgary, Calgary, AB, Canada

For all author emails, please log on.

BMC Cell Biology 2011, 12:53  doi:10.1186/1471-2121-12-53

Published: 14 December 2011

Additional files

Additional file 1:

Beating of bioreactor-derived cardiac body. The differentiation of mouse ESC into beating cardiac bodies was observed using video microcopy. The first beating EBs were seen on day 12 after LIF removal in stirred suspension bioreactor.

Format: MOV Size: 18.8MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data