Glycated albumin (GA) induces the up-regulation of CSF-1R and tyrosine-phosphorylation of CSF-1R in purified microglial cells. Microglial cells were incubated with or without GA for 24 h and then subjected to immunoprecipitation (IP) with antibodies against phospho-CSF-1R(Tyr723) and then blot with antibodies against phospho-CSF-1R(Tyr723) or CSF-1R. The expression of β-actin was used as loading control.
Liu et al. BMC Cell Biology 2011 12:5 doi:10.1186/1471-2121-12-5