Figure 1.

Effect of M-CSF, glycated albumin (GA) and the antibodies of either M-CSF(anti-M-CSF Ab)or CSF-1R(anti-CSF-1R Ab) on microglial viability. After incubated with various stimuli such as M-CSF or GA (A), and anti-M-CSF Ab (1 μg/ml) or anti-CSF-1R Ab (1 μg/ml) (B) for 24 h, the cell viability was assessed by MTT assay. The gradient concentrations of stimuli were: 5, 10, 25, 50, 100 ng/ml for M-CSF; and 10, 50, 100, 500, 1000, 2000 μg/ml for GA. The results are shown as means ± SD of three individual experiments. Only M-CSF induced microglial proliferation in a dose-dependent manner, other agents have no effects on microglial viability. One-way ANOVA was performed, followed by post-hoc analysis. *, P < 0.05; **, P < 0.005;

Liu et al. BMC Cell Biology 2011 12:5   doi:10.1186/1471-2121-12-5
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