Figure 4.

PGK1 released as a free soluble protein. Media was collected from HeLa cell cultures 7.5 min after serum withdrawal and then treated with (lanes 2 and 4) or without Proteinase K (PK, lanes 1 and 3) in the presence (lanes 3 and 4) or absence (lanes 1 and 2) of Triton X-100 (Triton) as described in Materials and Methods. Samples were separated on SDS-PAGE gels and then immunoblotted with anti-PGK1 antibodies.

Chirico BMC Cell Biology 2011 12:46   doi:10.1186/1471-2121-12-46
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