Additional file 4.
Movie S2. Pyronin Y labeling reveals a partial coalescence of RNA aggregates after 4 weeks of dehydration. Microspores collected and fixed after 4 weeks without watering were sectioned and double stained with DAPI and PY. Subnuclear PY (green) signal was detected in successive confocal slices and these were rendered as a 3D model. Movie is in .mov format playable with QuickTime.
Format: MOV Size: 116KB Download file
Boothby and Wolniak BMC Cell Biology 2011 12:45 doi:10.1186/1471-2121-12-45