Open Access Research article

Adhesion molecule periplakin is involved in cellular movement and attachment in pharyngeal squamous cancer cells

Yurie Tonoike12, Kazuyuki Matsushita23*, Takeshi Tomonaga34, Koji Katada12, Nobuko Tanaka2, Hideaki Shimada5, Yukio Nakatani6, Yoshitaka Okamoto1 and Fumio Nomura23

Author Affiliations

1 Department of Otorhinolaryngology, Chiba University Hospital, 1-8-1 Inohana, Chiba City, Chiba 260-8670, Japan

2 Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University Hospital, 1-8-1 Inohana, Chiba City, Chiba 260-8670, Japan

3 Department of Clinical Proteomics Research Center, Chiba University Hospital, 1-8-1 Inohana, Chiba City, Chiba 260-8670, Japan

4 Proteome Research Center, Proteome Research Project, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki City, Osaka 567-0085, Japan

5 Department of General and Gastrointestinal Surgery, Toho University Omori Medical Center, 6-11-1 Ohta-ku, Ohmori-nishi, Tokyo 143-8541, Japan

6 Department of Diagnostic Pathology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba City, Chiba 260-8670, Japan

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BMC Cell Biology 2011, 12:41  doi:10.1186/1471-2121-12-41

Published: 27 September 2011

Additional files

Additional file 1:

Adhesion assay of D562 cells.

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Additional file 2:

siRNA against PPL reduces adhesion to ECM in D562 cells. D562 cells were transfected with either control siRNA (Additional file 1) or PPL siRNA (Additional file 2). The cells were grown to confluence in glass-bottomed dishes and EDTA (2.5 mM final concentration) was added. Images were analyzed by time-lapse confocal laser scanning microscopy using a laser-scanning confocal microscope (LSM 510 META; Carl Zeiss Inc). Frames were taken every 5 min for 6 h.

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Additional file 3:

Migration of D562 cells.

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Additional file 4:

siRNA against PPL reduces cell migration in D562 cells. D562 cells were transfected with either control siRNA (Additional file 3) or PPL siRNA (Additional file 4). After 24 h, cells were seeded on glass-bottomed dishes and incubated for another 24 h. Images were analyzed by time-lapse confocal laser scanning microscopy using a laser-scanning confocal microscope. Frames were taken every 5 min for 24 h.

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Additional file 5:

pAktSer473 phosphorylation affects cellular movement in D562 cells. (A) (B) 5 × 105 YES5 cells (low PPL expression) and D562 cells (high PPL expression) were injected beneath right thigh of 8-week male nude mice and monitored subsequent tumor growth. No tumor growth was observed in YES5 mice, while some tumors were observed in D562 mice. (C) LY294002, a PI3K inhibitor, suppressed pAktSer473 phosphorylation but did not alter PPL expressio in D562 cells. (D) LY294002 suppressed D562 cell migration revealed by wound-healing assay in D562 cells. (E) The ratio of width at 6 hrs/0 hr (%) indicated that LY294002 suppressed wound-healing in D562 cells.

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