Open Access Research article

Sirtuin-mediated nuclear differentiation and programmed degradation in Tetrahymena

Kristin M Slade12, Sydney Freggiaro1, Kyle A Cottrell3, Joshua J Smith3 and Emily A Wiley1*

Author Affiliations

1 Keck Science Department of Claremont McKenna, Pitzer, and Scripps Colleges, W.M. Keck Science Center, 925 N. Mills Ave., Claremont, CA 91711, USA

2 Chemistry Department, Hobart and Williams Smith College, 300 Pultney St., Geneva, NY 14456, USA

3 Biomedical Sciences Department, Missouri State University, 901 S. National Ave., Springfield, MO 65897, USA

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BMC Cell Biology 2011, 12:40  doi:10.1186/1471-2121-12-40

Published: 21 September 2011

Additional files

Additional file 1:

"Time dependence of nicotinamide treatment effects". This data illustrates a decrease in the old macronucleus retention phenotype with NAM treatment later in conjugation.

Format: DOC Size: 312KB Download file

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Additional file 2:

"Thd14 sirtuin core domain resembles that of other class I sirtuins". This is an alignment of Thd14 with the sirtuin domains of yeast and human enzymes. It shows strong conservation of active site residues and metal binding residues.

Format: DOC Size: 36KB Download file

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Additional file 3:

"Thd14 has histone deacetylase activity". This is data from an experiment testing for histone deacetylase activity. It shows that Thd14 deacetylates histone amino-terminal peptides.

Format: DOC Size: 47KB Download file

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