Additional file 7.
Time lapse imaging of H+ fluxes at the cell periphery. pH bubble formation at the termini of membrane protrusions on the leading-edge of cathode-directed (Calvaria) cells during electrotaxis. Cells were loaded with BCECF-AM, a pH reporter, ratiometric dye. Images were collected every 9 sec for 25 min using inverted fluorescence microscopy.
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Özkucur et al. BMC Cell Biology 2011 12:4 doi:10.1186/1471-2121-12-4