Additional file 3.

Characterisation of anti-Notch antibody. (A-C) Western Blot after SDS-PAGE of lysates from E.coli expressing myc tagged HvNΔE (49 kDa) from pRSET probed with anti-Notch antibody (A), probed with chicken preimmune serum (B), probed with anti-myc antibody (C); arrows indicate HvNΔE and two apparent degradation products; (D-G) HEK293T cells expressing HvNotch-GFP from pcDNA3. (D) DNA staining with TO-PRO3, (E) HvNotch-GFP, (F) anti-Notch antibody staining (G) merged images in false colours: DNA (blue), HvNotch-GFP (green), anti-Notch staining (red); Confocal sections; scale bar: 5 μm.

Format: TIFF Size: 13MB Download file

Prexl et al. BMC Cell Biology 2011 12:38   doi:10.1186/1471-2121-12-38