Figure 13.

Co-immunofluorescence staining of Hydra whole mount with anti-JAG-IC- and anti-Notch-antibodies. Upper panel left: schematic representation of an adult hydra, the region imaged in lower panel is indicated; upper panel, right: schematic drawing of cells that were imaged including ectodermal epithelial cells, interstitial cells, nematoblasts, nematocytes and nerve cells; positions of confocal sections are boxed in red (A-D) and green (E-H). (A, E) DNA staining with TO-PRO3, (B, F) anti-JAG-IC staining, (C, G) anti-Notch staining, (D, H) merged images in false colours: DNA (blue), anti-JAG-IC staining (green), anti-Notch staining (red); single confocal sections; scale bars: 20 μm.