Levels of phosphorylated H2A.X (γH2A.X) were determined using ELISA for the RMS cell lines RD, RH30, and Res30 following exposure to 20 nM of AS-DACA over time. Levels were calculated as percentages of γH2AX detected after 48 h-exposure to 4 μM AS-DACA. Error bars are SEM for two experiments performed in triplicate providing six independent readings per value.
Wolf et al. BMC Cell Biology 2011 12:36 doi:10.1186/1471-2121-12-36