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Open Access Research article

Localization of chondromodulin-I at the feto-maternal interface and its inhibitory actions on trophoblast invasion in vitro

Shigenori Miura1, Chisa Shukunami1, Kaori Mitsui2, Jun Kondo3 and Yuji Hiraki1*

Author Affiliations

1 Department of Cellular Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan

2 Research and Development Division, Science and Technology Research Center Inc., Mitsubishi Chemical Group, Kanagawa 227-8502, Japan

3 Advanced Medical Research Laboratory, Research Division, Mitsubishi Tanabe Pharma Corporation, Kanagawa 227-0033, Japan

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BMC Cell Biology 2011, 12:34  doi:10.1186/1471-2121-12-34

Published: 18 August 2011

Abstract

Background

Chondromodulin-I (ChM-I) is an anti-angiogenic glycoprotein that is specifically localized at the extracellular matrix of the avascular mesenchyme including cartilage and cardiac valves. In this study, we characterized the expression pattern of ChM-I during early pregnancy in mice in vivo and its effect on invasion of trophoblastic cells into Matrigel in vitro.

Results

Northern blot analysis clearly indicated that ChM-I transcripts were expressed in the pregnant mouse uterus at 6.5-9.5 days post coitum. In situ hybridization and immunohistochemistry revealed that ChM-I was localized to the mature decidua surrounding the matrix metalloproteinase-9 (MMP-9)-expressing trophoblasts. Consistent with this observation, the expression of ChM-I mRNA was induced in decidualizing endometrial stromal cells in vitro, in response to estradiol and progesterone. Recombinant human ChM-I (rhChM-I) markedly inhibited the invasion through Matrigel as well as the chemotactic migration of rat Rcho-1 trophoblast cells in a manner independent of MMP activation.

Conclusions

This study demonstrates the inhibitory action of ChM-I on trophoblast migration and invasion, implying the potential role of the ChM-I expression in decidual cells for the regulated tissue remodeling and angiogenesis at feto-maternal interface.