Additional file 4.
MTX treatment in NIH3T3 cells (A) A FACS analysis of the control, 2 days or 9 days of MTX treated cells stained with propidium iodide. (B) The percentage of untreated or MTX treated NIH3T3 cells in different phases of the cell cycle analyzed by using ModFit V 3.0 software. (C) Western blotting analysis of the nucleolar, nucleoplasmic and cytosolic lysate from MTX (9 days) treated NIH3T3 cells with a specific antibody towards a nucleolar (anti-B23), nucleoplasmic (anti-Lamin A/C) or cytoplasmic (anti actin) protein marker. The protein concentration of the lysates from the different cellular fraction were determined using Bradford-Lowry reagent and 10 ug of lyastes from each fraction were run on a SDS-PAGE.
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kar et al. BMC Cell Biology 2011 12:33 doi:10.1186/1471-2121-12-33