Induction of senescence in NIH3T3 cells following sodium butyrate treatment (A) NIH3T3 cell was treated with 5 mM of sodium butyrate for 4 days and the morphological changes were monitored under a microscope. The bar represents 10 μm. (B) A FACS analysis of the control and treated NIH3T3 cells followed by propidium iodide staining to analyze the cell cycle progression. Percentage of untreated or sodium butyrate treated NIH3T3 cells in different phases of the cell cycle analyzed by using ModFit V 3.0 software. (C) A graphical representation of the percentage of cells in different cell cycle phases in control and Sodium Butyrate treated cells as determined by propidium iodide staining and followed by FACS analysis. (D) SA-β-gal staining of the control and sodium butyrate treated NIH3T3 cells following 4 days of treatment. Blue coloration in cells indicates senescence.
kar et al. BMC Cell Biology 2011 12:33 doi:10.1186/1471-2121-12-33