Seeding of bone marrow cells directly onto MEF-ECM improves isolation of primary mMSCs with normal ploidy. (A) Karyotype analysis of primary mMSCs cultured on plastic or MEF-ECM, at 2% O2 with addition of antioxidants. (B) The isolated mMSCs show differentiation potentials into typical cell lineages. Adipogenesis, osteogenesis, and chondrogenesis were indicated by Oil Red, Alizarin Red (pH 4.1) and Alcian blue, respectively.
Fan et al. BMC Cell Biology 2011 12:30 doi:10.1186/1471-2121-12-30