Antioxidants increase proliferation of mMSCs. (A) Comparison of spontaneous formation of micronucleus in primary mMSCs supplemented with or without antioxidants (800 μM PBN+5 mM NAC). (B) Effects of antioxidants on number of mMSCs. Bone marrow cells were plated at a density of 2 × 105 cells/cm2 on 6-well and cultured under 2% O2 with or without addition of antioxidants for 5 days, and cells were counted by hemocytometer. (C) Effects of H2O2 on the proliferation of mMSCs. Primary mMSCs were replated at 1000 cells/cm2 and cultured for two days, then H2O2 were added and cell number counted after 24 h. (D) Micronucleus analysis on mMSCs. (E) Karyotype analysis of mMSCs on plastic or ECM treated with H2O2. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01; n = 3. (F) A representative proliferation curve of mMSCs from three independent experiments. Data are expressed as the mean ± SD. *p < 0.05; n = 3. Abbreviations: Con., control; PN, PBN+NAC; CmMSCs, mMSCs on plastic as controls; EmMSCs, mMSCs on MEF-ECM; PCmMSCs, mMSCs on plastic in the presence of PBN and NAC; PEmMSCs, mMSCs on MEF-ECM in the presence of PBN and NAC.
Fan et al. BMC Cell Biology 2011 12:30 doi:10.1186/1471-2121-12-30