Purification of mMSCs by culture under 2% O2 condition. (A) Flow cytometry analysis of epitopes on primary mMSCs. Few cells show CD45 and CD11b antigens by culture under 2% O2 compared to under 20% O2. (B) Flow cytometry analysis of epitopes on mMSCs at passage one. CmMSCs show epitopes similar to IDmMSCs. (C) Differentiation of CmMSCs. Adipogenesis, osteogenesis, and chondrogenesis were indicated by Oil Red, Alizarin Red (pH 4.1) and Alcian blue, respectively. Scale bar = 100 μm.
Fan et al. BMC Cell Biology 2011 12:30 doi:10.1186/1471-2121-12-30