Table 2

Location of the target sequences within topological zones relative to the NM

TOPOLOGICAL ZONES RELATIVE TO NM ACCORDING TO KINETICS OF DNA DIGESTION OF NUCLEOIDS FROM GO


AMPLICON

HEPATOCYTES


CONTROL G0

PHX-24

PHX-7 D


C

D

P

VC

E

C

D

P

VC

E

C

D

P

VC

E


a

+

+

+

-

-

+

+

+

+

-*

+

+

-*

-

-

b

+

+

-

-

-

+

+

+

+*

-

+

+

-

-

-

c

+

+

-

-

-

+

+

+

-*

-

+

+

-

-

-

d

+

+

+

-

-

+

+

+

+

-

+

+

+*

-

-

e

+

+

-

-

-

+

+

+

+*

-

+

-*

-

-

-

f

+

+

-

-

-

+

+

+

+

-

+

+

-

-

-

g

+

+

-

-

-

+

+

+

-*

-

+

+

-*

-

-

h

+

+

+

-

-

+

+

+

+

-

+

+

-*

-

-

i

+

+

-

-

-

+

+

+

+

-

+

+

-

-

-

j

+

+

-

-

-

+

+

+

+

-

+

+

-*

-

-

k

+

+

+

-

-

+

+

+

+

-*

+

+

-*

-

-

l

+

+

-

-

-

+

+

+

+*

-

+

+

-

-

-

m

+

+

+

-

-

+

+

+

+*

-

+

+

+*

-

-

n

+

+

-

-

-

+

+

+

+*

-

+

+

-

-

-

o

+

+

+

-

-

+

+

+

+

-*

+

+

+*

-

-


Location of the fifteen target sequences from the 162 kbp genomic region studied within the topological zones relative to the NM. Such topological zones were defined according to the kinetics of nucleoid-DNA digestion in samples from control G0 hepatocytes obtained from normal rat liver (Figure 2A). The amplicons were scored either as positive or negative as a function of endonuclease digestion time and for each topological zone relative to the NM, depending on whether or not they were detected by a digital image-analysis system (Kodak 1D Image Analysis Software 3.5) using the default settings. G0, samples from normal rat liver hepatocytes. PHx-24, samples obtained from hepatocytes 24 h after partial hepatectomy. PHx-7 D, samples obtained from hepatocytes 7 days after partial hepatectomy. C, 0' digestion-time control. D, distal to the NM. P, proximal to the NM. VC, very close to the NM. E, embedded within the NM. All target sequences were mapped in repeated experiments with samples from separate animals (n ≥3). *Indicates that one of the repetitions gave a different result: -* in one experiment was positive; +* in one experiment was negative.

Rivera-Mulia et al. BMC Cell Biology 2011 12:3   doi:10.1186/1471-2121-12-3

Open Data