Modulation of lymphatic vessel outgrowth by protease inhibitor. Lymphatic rings cultured in the presence of serum were treated with a broad spectrum MMP inhibitor (GM6001) at 0.1 μg/ml and 0.001 μg/ml. Quantification of LEC sprouting was measured by determining the number of intersections between capillaries and a grid obtained by the dilatation of the ring boundary, as previously described . The number of intersections (Ni) is plotted as a function of the distance to the ring. * = P < 0.05.
Detry et al. BMC Cell Biology 2011 12:29 doi:10.1186/1471-2121-12-29