Figure 1.

Lymphangiogenesis in vivo. (A-C): Corneal lymphangiogenesis induced by thermal cauterization. Corneal flatmounts are labeled in green with an anti-LYVE-1 antibody and observed on fluorescent (A, B) or confocal (C) microscope. (A): In normal condition, cornea is avascular and the limbal vascular arcade is positive for LYVE-1 staining. (B, C): Seven days after an inflammatory stimulus, lymphatic vessels outgrow from the limbus towards the central cornea. (C): Migrating LEC display filopodia-like structures. (D-F): Mouse lymphangioma induced by intraperitoneal injection of incomplete Freund's adjuvant. (D): Lymphangiomas appear as white masses at the surface of the diaphragm (white arrowheads). (E): Hematoxylin-eosin staining of a histological section of the diaphragm (black arrowheads: lymph vessels) (F): Lymphatic vessels are evidenced by LYVE-1 immunostaining. The arrow delineated the process of fusion leading to increased lumen size. Scale bars in (A, B): 1 mm, in (C): 20 μm, and in (E, F): 100 μm.