Table 3

Lateral mobility of the flotillin-2 mutant lacking the actin binding domain (R1MCT) expressed in freshly isolated T-lymphocytes without or with wild type flotillin-1-mCherry

Transfection

MF - SDF-1 (n)

MF + SDF-1 (n)


R1MCT

0.72 ± 0.05 (15)

0.79 ± 0.06 (14)

R1MCT/flo1-mCherry

0.65 ± 0.06 (14)

0.79 ± 0.05 (15)


FRAP analysis involving local bleaching of EGFP was carried out as described in the legend of Figure 6 with cells preincubated in the absence or presence of SDF-1 as indicated. The mobile fraction (MF) was calculated for each individual cell and then averaged from a total of n = 14 - 15 cells observed in 3 independent experiments, as indicated (mean ± s.e.m.).

Affentranger et al. BMC Cell Biology 2011 12:28   doi:10.1186/1471-2121-12-28

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