Table 1

FRAP analysis of the lateral mobility of flotillins in T-lymphocytes

Transfection

Cell type

MF - SDF-1 (n)

MF + SDF-1 (n)


flo1-EGFP

T-lymphoblast

n.d.

0.88 ± 0.05 (12)

flo1-EGFP/flo2-mCherry

T-lymphoblast

n.d.

0.04 ± 0.01 (10)

flo2-EGFP

T-lymphoblast

n.d.

0.80 ± 0.06 (7)

flo2-EGFP/flo1-mCherry

T-lymphoblast

n.d.

0.06 ± 0.02 (7)

flo2-EGFP

Freshly isolated T-cells

0.79 ± 0.04 (14)

0.71 ± 0.03 (15)

flo2-EGFP/flo1-mCherry

Freshly isolated T-cells

0.38 ± 0.04 (16)

0.11 ± 0.02 (11)


FRAP analysis involving local bleaching of EGFP was carried out as described in the legend of Figure 6 with cells preincubated in the absence or presence of SDF-1, as indicated. The mobile fraction (MF) was calculated for each individual cell and then averaged from a total of n = 7-16 cells, as indicated (mean ± s.e.m.) (data obtained in 2-4 independent experiments). N.d. = not done.

Affentranger et al. BMC Cell Biology 2011 12:28   doi:10.1186/1471-2121-12-28

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