Histological analysis of muscle after injury. Sections of the gastrocnemius muscle before (A) and after trauma stained with Toluidin blue reveal invasion of mononucleated cells after 1 day (B) and massive regeneration after 4 days (C). 10 days after injury (D) regeneration is nearly completed and multiple regenerated fibers are visible. Magnification is 200×. (E) Expression analysis of Myogenin showing highest expression levels after 4 days of injury (n = 6 control animals, n = 2 animals at day 1 and n = 3 animals at day 4 and day 10, respectively).
Friedrichs et al. BMC Cell Biology 2011 12:26 doi:10.1186/1471-2121-12-26