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BMP signaling balances proliferation and differentiation of muscle satellite cell descendants

Melanie Friedrichs12, Florian Wirsdöerfer3, Stefanie B Flohé3, Sabine Schneider1, Manuela Wuelling1 and Andrea Vortkamp1*

Author Affiliations

1 Center for Medical Biotechnology, Faculty of Biology, Department of Developmental Biology, University of Duisburg-Essen, D-45117 Essen, Germany

2 Department of Paediatrics, University Hospital of Essen, D-45147 Essen, Germany

3 Surgical Research, Department of Trauma Surgery, University Hospital Essen, D-45147 Essen, Germany

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BMC Cell Biology 2011, 12:26  doi:10.1186/1471-2121-12-26

Published: 6 June 2011

Additional files

Additional file 1:

Figure S1 BMP signaling does not alter cell cycle kinetics of myoblasts. Myoblasts were differentiated in control medium (A-C,C) or in medium supplemented with Bmp7 (D-F,F) or Noggin (G-I,I) for 3 days and labeled for two hours with BrdU at the end of the cultivation phase. (J) No statistically significant alteration of the percentage of cells in S-phase within the pool of mononucleated myoblasts could be detected after Bmp7 (p = 0.11) or Noggin (p = 0.66) treatment (n > 6 individual cultures per treatment from 2 independent experiments). Boxed areas in (C,F,I) are enlarged in (C',F',I'). Magnification is 100×.

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