Figure 3.

Specific labelling of AP-1 and GGA in Drosophila testes. Spermatocytes were triple labelled for α-tubulin (sperm tails and cytoplasmic microtubules), Hoechst (to determine the stage in sperm development) and either (A) AP-1, (B) GM130, and (C) GGA. Note that AP-1, GGA and GM130 all localise to large peripheral puncta reminiscent of Golgi mini stacks. (D) Spermatocytes from GGA knockdown flies were triple labelled for α-tubulin, Hoechst and GGA. Note that there is loss of the punctate cytoplasmic GGA labelling, demonstrating the specificity of the GGA antibody (the knockdown flies are further characterised in Figures 6 and 7) Bar, 20 μm

Hirst and Carmichael BMC Cell Biology 2011 12:22   doi:10.1186/1471-2121-12-22
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