Surface expression of ADAM10 is increased by dyn I K44A overexpression. HEK-M3 cells were transiently transfected with ADAM10, dyn I K44A, or empty vector as indicated. After 48 h, cells were surface-biotinylated and lysed. Biotinylated proteins and cell lysates were analyzed by immunoblotting. A. Bands representing biotinylated immature (im) and mature (m) ADAM10 on the cell surface are indicated by arrows (upper panel). Cell lysates were immunoblotted with antibodies to ADAM10 (middle panel) and dynamin I (lower panel). B. Surface levels of mature and immature ADAM10 in cells co-expressing ADAM10 and dyn I K44A were quantitated and normalized relative to levels in cells co-expressing ADAM10 and empty vector. Results are expressed as means ± SEM from five experiments. *Significantly different from ADAM/vector control.
Carey et al. BMC Cell Biology 2011 12:20 doi:10.1186/1471-2121-12-20