Additional file 4.


Time-lapse imaging of Fucci2-expressing HeLa cells during etoposide treatment. HeLa/Fucci2 cells were grown on a glass-bottom dish and time-lapse imaging was performed using an LCV100. Cells were treated with 1 μM etoposide. Cells at 4 different positions were imaged every 20.5 m. Playback speed is 7,000 × real time. Total imaging time = 49 h. Most cells were arrested at the G2 DNA checkpoint (yellowish green).

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Sakaue-Sawano et al. BMC Cell Biology 2011 12:2   doi:10.1186/1471-2121-12-2