Co-immunoprecipitations of MMGL isoform 4 and the C1-C2 region of cMyBPC. A. In vitro co-immunoprecipitation showing that MMGL interacts with the native as well as the trisphospho-mimic of C1-C2 in the absence of Y2H GAL4 domains. B. In vivo co-immunoprecipitation, showing that dsRed-tagged MMGL is able to specifically pull down GFP-cMyBPC, and vice versa, in H9C2 cells. Abbreviations: JL8 = antibody directed against GFP, dsRed = antibody directed against dsRed-tagged proteins, HA = antibody directed against haemaglutinin protein, used as negative control antibody, IP = antibody used in immunoprecipitation, WB = antibody used in western blotting.
Uys et al. BMC Cell Biology 2011 12:18 doi:10.1186/1471-2121-12-18