HDGF-S103A is not a mitogen. HDGF-GFP-S103A was expressed in mouse VSMC for 24 hours and cells labeled with BrdU overnight. Cells were immunostained for BrdU as a marker of DNA replication and observed for co-localization of GFP and BrdU. A = cells expressing HDGF-GFP-S103A, B = no staining for BrdU, C = DAPI staining to show that HDGF-GFP-S103A nuclear targeting is not affected.
Everett et al. BMC Cell Biology 2011 12:15 doi:10.1186/1471-2121-12-15