HDGF S103 is phosphorylated during M phase of the cell cycle. (A) Representative immunostaining for phospho-S103 HDGF was first detected in COS-7 cells that have undergone nuclear envelope breakdown and chromosomal condensation, (B) and in cells in metaphase and early anaphase. (C and D) HDGF-S103 phosphorylation rapidly falls to undetectable levels as daughter cells separate in late anaphase and telophase. Co-staining with DAPI demonstrates chromosomal DNA condensation in phospho-S103-HDGF immunostained cells (Merge).
Everett et al. BMC Cell Biology 2011 12:15 doi:10.1186/1471-2121-12-15