Phospho-S103-HDGF Immunoblotting. Western blot of COS-7 whole cell lysate (25 ug, Cell lysate), cell lysate PhosphoProtein column flow through (25 ug) or PhosphoProtein column bound proteins (25 ug, Po4 proteins, PhosphoProtein Purification Kit, see Methods) using a specific anti-phospho-S103-HDGF (top panel, A) or pan-anti-HDGF (bottom panel, B). Arrows = the high and low molecular weight bands in the Po4 protein lane with both antibodies.
Everett et al. BMC Cell Biology 2011 12:15 doi:10.1186/1471-2121-12-15