Table 1

γTub37C kinetic parameters in the spindle and cytoplasm

Spindle or cytoplasm

D(μm2/s)

k*on (s-1)

koff (s-1)

t1/2 (s)

Ceq


MI spindle

12 ± 5

0.02 ± 0.02

0.6 ± 0.3

1.1

0.03 ± 0.03

Ooplasm

8 ± 3

0.14 ± 0.03

0.9 ± 0.1

0.8

0.14 ± 0.03

Mitotic spindle

28 ± 10

0.015 ± 0.006

0.23 ± 0.04

3.0

0.06 ± 0.02

Embryo cytoplasm

26 ± 8

0.009 ± 0.004

0.15 ± 0.03

4.5

0.05 ± 0.02


Kinetic parameters from fits to the FRAP data of the conical reaction-diffusion model [32]; the half-time of recovery was calculated by t1/2 = ln2/koff and the bound protein fraction at equilibrium by Ceq = k*on/(k*on+koff) [31].

Endow and Hallen BMC Cell Biology 2011 12:1   doi:10.1186/1471-2121-12-1

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