Western blot analysis of the level of hRev7 protein. The level of hRev7 protein was examined by Western blotting of nuclear extracts obtained from the parental cell strain (P), derivative strains expressing hRev7 siRNA (clones 2-2, 2-6, 2.5 and 3.2), control strains expressing an siRNA with limited homology to known sequences in the human genome (VCA and V1.1), as well as a complementation derivative of cell strain 2-2 that expresses an siRNA insensitive hRev7 (2-2 + R7). Ku80 was used as a loading control.
Neal et al. BMC Cell Biology 2010 11:97 doi:10.1186/1471-2121-11-97