Additional file 2.
Isolated lipid droplets free of LRO marker protein GLO-1::GFP. Lipid droplet fraction and cytoplasm fraction were prepared from stage L4 dhs-28;glo-1::gfp animals. Triplicates. Fractions were probed for the presence of GLO-1::GFP protein using a GFP antibody. GLO-1::GFP (predicted MW, ~50 kD, arrow) was detected in cytoplasm fractions but not in lipid droplet fractions. Asterisk denotes a non-specific protein recognized by the GFP antibody that is present in wild-type control (WT).
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Zhang et al. BMC Cell Biology 2010 11:96 doi:10.1186/1471-2121-11-96