Figure 4.

Post-fix Nile Red staining labels lipid droplet-specific Spec-2 structures but not LRO-specific Spec-1 structures. (A) Nile Red labelled structures in fixed wild-type animals as revealed by channel mode confocal images. (B) These labelled structures displayed lipid droplet-specific Spec-2 but not Spec-1 spectrum as revealed by linear un-mixing lambda mode confocal images. (C) Nile Red labelled structures in fixed glo-4 animals as revealed by channel mode confocal images. (D) These labelled structures displayed lipid droplet-specific Spec-2 but not Spec-1 spectrum as revealed by linear un-mixing lambda mode confocal images. Arrows point to structures stained by Nile Red in hypodermal cells. E. Emission spectrum profiles of Spec-2s in post-fixed wild-type and glo-4 animals. All images were single confocal slices. Scale bars, 10 μm.