Effects of PKA activity on respiratory function and ROS production. We analysed the influence of individual subunits on respiratory function by measuring oxygen consumption in strains lacking combinations of PKA subunit gene deletions (A). The cAMP phosphodiesterase PDE2 was also deleted to allow elevation of cAMP levels by addition of exogenous cAMP (4 mM). ROS production in response to cAMP elevation was analysed in strains expressing only one of each of the three PKA subunits, Tpk1p, Tpk2p or Tpk3p (B). All strains were grown for 24 h to the diauxic phase of growth.
Leadsham and Gourlay BMC Cell Biology 2010 11:92 doi:10.1186/1471-2121-11-92