NK cell cytotoxicity towards HK-2 cells was measured by LDH release assay kit. HK-2 cells were collected 96 h after transduction of Ad.CMV.HIF-1alpha, Ad.CMV.HIF-1alphaDELTAODD, Ad.CMV.LacZ and 8 h after hypoxia/reoxygenation with/without RNAi treatment. Before cells were used in the cytotoxicity assay, a blocking mAb was added or not. (A) No antibody, (B) isotype-matched mAb, (C) blocking MICA mAb. LDH measurements were made in triplicate from three separate assays. Values are shown as means ± SD from three independent experiments. *indicates a significant difference compared with the control group (p < 0.05).
Luo et al. BMC Cell Biology 2010 11:91 doi:10.1186/1471-2121-11-91