Transduction of HIF-1alpha siRNA can inhibit HIF-1alpha and MICA expression on HK-2 cells during hypoxia/reoxygenation. The relative mRNA levels were determined by real time PCR 0, 4, 8, and 16 h after reoxygenation and normalized to the housekeeping gene β-actin. (A) Relative mRNA levels of HIF-1alpha. (B) Relative mRNA levels of MICA. Values are shown as means ± SD from three independent experiments. *indicates a significant difference compared with the control group (p < 0.05).
Luo et al. BMC Cell Biology 2010 11:91 doi:10.1186/1471-2121-11-91