Immunofluorescence of PINK1-MLS GFP, Δ151 PINK1, mito-Δ151 PINK1, and Immt-Δ151 PINK1 in Hela cells. Cells transiently transfected with aforementioned constructs are also co-stained with either cytosol marker Hsp90 or mitochondria marker COX IV. Confocal images are analyzed with ImageJ and the yellow color in the overlay panels represents co-localization. Scale bar = 5 μm.
Lin and Kang BMC Cell Biology 2010 11:90 doi:10.1186/1471-2121-11-90