Expression of Atg8b-GFP with Dcp-1 in S2 cells. S2 cells were transfected with Atg8b-GFP with or without Dcp-1 constructs were maintained under (A) FBS supplied or (B) starvation-inducing culture conditions. (C) Atg8b-GFP was induced with full-length (fl-) Dcp-1 in the presence of FBS. (D) Atg8b-GFP was induced with truncated (t-) Dcp-1. (E) The percentage of cells with multi-autophagosomes was counted by cell number of punctate Atg8b-GFP expression. (n of; control: 1,705, 2 hour starvation: 1,353, 4 hour starvation: 1,464, 6 hour starvation: 1,304, t-Dcp-1: 1,019, fl-Dcp-1:1,031, (*, P < 0.05; **, P < 0.01 t test). Starvation is abbreviated to ST. Scale bars in A-D stand for 10 um. We also tested the expression level of Dcp-1 and Atg8b in the lines that over-express Dcp-1 with Atg1, Atg6, Aut1 and S6kwt but we found that the gene expression levels were not significantly affected in any case (Additional file 7).
Kim et al. BMC Cell Biology 2010 11:9 doi:10.1186/1471-2121-11-9