Suppressors of the Dcp-1 GF eye phenotype. (A) Dcp-1 GF, The Dcp-1 eye phenotype of flies with a single copy of Dcp-1 GF was suppressed by: (B) Atg2EP(G6691), (C) Atg6EP(G6854), (D) Atg1EP(G13748), (E) Aut1EP(G3894), (F) UAS-dS6k, (G) Tork17004, (H) SNF4AγEP(GX6409), (I) Eip55EEP(G13564), (J) brEP(G10174), (K) Ubp64EEP(G5032), and (L) Uba2EP(G4384). Each panel shows the effects of each gene in the Dcp-1 GF background. GD- indicates DCP-1 expression with each transgenes by crossing with Dcp-1 GF. (M) Caspase activity was determined with lysate from heads of each virgin flies from w1118, GMR-GAL4;UAS-Dcp-1 and GMR-GAL4;UAS-Dcp-1 which were crossed with autophagy related lines. Samples that provided statistically significant reduced activity in at least three independent experiments are shown (*, P < 0.05; **, P < 0.1 t test). RLU, relative light unit. The error bars represent the standard deviation of amount of RLU (B, C, D and E).
Kim et al. BMC Cell Biology 2010 11:9 doi:10.1186/1471-2121-11-9