Table 2

Early apoptosis events in U937 cells after the freezing - thawing cycle.

Time after thawing

The devices used for FTC

Untreated cells*


i3C

standard cryo-vial


3 hours

4.3% ± 2.1 (3% - 7%)

9.9% ± 6.2 (1% - 19%)


overnight

10.0% ± 10.0 (2% - 20%)

3.0% ± 2.8 (1% - 5%)

7.0% ± 4.3 (1.5% - 10%)


U937 cells underwent a freezing - thawing cycle as described in Materials and Methods. The percentage of early apoptotic (Annexin V positive and PI negative) cells was tested at appropriate time points after thawing. Values are average ± SD of 2-3 experiments (500-1000 individual cells). The range of results obtained for the different experiments (as percentages) is presented in brackets. *Untreated U937 cells, which did not undergo FTC, were evaluated at the corresponding time points.

Afrimzon et al. BMC Cell Biology 2010 11:83   doi:10.1186/1471-2121-11-83

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