Table 1

Cell death in U937 cells after the freezing - thawing cycle.

Time after thawing

The devices used for FTC


i3C

mini-vial

Standard cryo-vial


2 × 106 cells/ml

10 × 106cells/ml


1 hour (after-80°C)

8.7% ± 3.5

9.1% ± 5.6


3 hours (after-80°C)

9.2% ± 5.1

6.3% ± 3.2

12.5% ± 4.1


overnight (after-80°C)

23.4% ± 23.5

11.6% ± 2.3

19.6% ± 10.1

17.5% ± 11.3


overnight (after-196°C)

18.5 ± 2.9

24.6 ± 7.0


U937 cells underwent a freezing - thawing cycle as described in Materials and Methods. The percentage of dead (PI positive) cells was tested at appropriate time points after thawing. The corresponding temperature end points are presented. Values are average ± SD of 2-3 experiments (500-1000 individual cells).

Afrimzon et al. BMC Cell Biology 2010 11:83   doi:10.1186/1471-2121-11-83

Open Data