Relative genes expression of early passage senescent BMECs, BME65Cs and MCF-7 cells. A. The detection of relative genes expression by RT-PCR. B. Analysis of expression level by gray scale. The expression level of each gene in BMECs cells (PDL 14) was set to 1.0. The expression level for TRPS-1, Bag-1 and c-Myc in MCF-7 cells was set to 1.0 because these three genes expressions were not detected in BMECs cells (PDL 14)]. Other samples were adjusted accordingly. All PCR products were confirmed by sequencing. Gray scale analysis is from at least 3 independent experiments. *: P < 0.05 compared to BMECs cells passage 14 (Student's t-test). **: P < 0.01 compared to BMECs cells passage 14 (Student's t-test).
Zhao et al. BMC Cell Biology 2010 11:82 doi:10.1186/1471-2121-11-82