Detection of bTERT expression and analysis of telomere length in BME65Cs and BMECs cells. A. The detection of bTERT mRNA by RT-PCR. The bovine catalytic subunit of telomerase (bTERT) mRNA levels from primary early passage 5, BMECs cells senescent passage 22 and immortal BME65Cs cells passage 104 were determined by RT-PCR using sequence-specific primer pairs. β-actin was used as a loading control. B. Southern-blot analysis of mean telomeric length. Genomic Southern-blot analysis was performed to determine the telomeric lengths of primary early passage 5 and senescent passage 22 BMECs as well as immortal BME65Cs cells passage 104. The DNA marker (λ DNA/Hind III) was used to determine the telomere length of various samples.
Zhao et al. BMC Cell Biology 2010 11:82 doi:10.1186/1471-2121-11-82