Soft agar assay and karyotype analysis in immortal BME65s cells and early passage BMECs cells. A. Analysis of colony formation used for evaluating malignant potential by the soft agar assay. Colonies containing more than ten viable cells were scored positive. Obvious colony formation was shown in MCF-7 cells, which served as a positive control. Bars: 50 μm. B. Comparison of chromosome numbers. At least 20 well-spread metaphase chromosomes from immortal BME65Cs cells (passage 110) and BMECs cells (primary passage16) were examined and the characteristic patterns illustrated were observed.
Zhao et al. BMC Cell Biology 2010 11:82 doi:10.1186/1471-2121-11-82