Figure 6.

Change of the nuclear organization of MLP29 hepatic progenitor cells. Quantitative FISH was performed with a pan-centromeric probe. Computational analysis of the spatial organization of chromocenters showed marked variations in their average volume, whereas the nuclear volume remained unchanged in MLP29 cells following α₅β₁ blockade or stimulation with HGF/SF1. 3D computational reconstruction of the image stacks and quantitative analysis showed that the average fluorescence intensity of the Me3H3K9 foci remained unaltered. However, the number of foci increased concomitantly with a reduction of their volume after both types of treatments. Immunofluorescent detection of Ac3K9/14 H3, on the other hand, revealed that cell scattering is associated with an increase in the level of acetylation of histone H3 at K9.